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技术文章

app平台赚钱项目 >> 技术文章

牛奶中β-内酰胺酶检测方法
发布时间:2013.07.16 点击次数: 次

“WUKANGNAI”,GUMINGSIYI,JIBUHANKANGSHENGSUDENIUNAI。ZAOZAI2002NIAN,ZHEIYIGAINIANBIANBEITICHU,“WUKANGSHENGSU”DEZIYANGYEDENGSHANGLERUPINBAOZHUANG。

WEIHENIUNAIHANKANGSHENGSU?

NIUNAIZHONGDEKANGSHENGSULAIZIRUNIU,RUNIUBEIZHUSHEKANGSHENGSU,ZHUYAOSHIZHILIAORUXIANFAYAN。RUXIANYANSHINAINIUCHANGJIANBING,KANGSHENGSUDESHIYONGSHIYENEI“GONGKAIDEMIMI”。NIUNAIZHONGDEKANGSHENGSUKENENGDAOZHIYINYONGZHEGUOMIN,HUOYINZHANGQIJIECHUKANGSHENGSUERNAIYAO。

RUHEZUODAO“WUKANG”?

YAOPINHELIJIANGEQIHOUCAIJINAI,KEYISHENGCHANWUKANGNAI,KEYITONGGUOJIANYANKANGSHENGSUDEFANGSHIYANZHENG。

1、JIANJIEFA

FANGFAYUANLI:LIYONGβ-NEIXIANANMEINENGGOUMEIJIEQINGMEISUDEYUANLI,XIANGNIUNAIZHONGTIANJIAYIDINGLIANGDEQINGMEISU,RUGUONIUNAIZHONGCUNZAIYIDINGNONGDUDEβ-NEIXIANANMEI,NEIMEQINGMEISUJINGβ-NEIXIANANMEIMEIJIEHOUNONGDUHUIJIANSHAO,CONGERPANDUANNIUNAIZHONGSHIFOUCUNZAIβ-NEIXIANANMEI。

SHIYANBUZHOU:CHENGQU20 gSHIYANG,ZAI4℃、16000rpmTIAOJIANXIALIXIN10 min。QUXIACENGQINGYE10 gYU50

mLSULIAOLIXINGUAN,BINGJIANGSULIAOLIXINGUANZHIYU37℃SHUIYUGUOZHONGZHENDANGFUYU30 min。XIANGFUYUHOUDELIXINGUANZHONGJIARUWUSHUIYICHUN15 mL。ZHENDANGTIQU30

minHOULIXIN,JIANGSHANGCENGQINGYEGUOLVZHIHOUSHOUJIYULIXINGPINGZHONG。JIANYANONGSUOZHENGFADIAOYICHUN。XIANGXUANZHENGHOUDELIXINGPINGZHONGJIARU10 mLLINSUANYANHUANCHONGYE(pH=8.5),WOXUAN1

minHOUDIAOJIEpHWEI8.5。YI1 mL/minDESUDUJIANGTIQUYETONGGUOJINGGUOYUCHULIDEOasis HLBGUXIANGCUIQUZHU,YONG2 mLLINSUANHUANCHONGYE(pH=8.5)LINXICUIQUZHU,ZAIYONG2

mLSHUILINXI。ZUIHOUYONG3 mLYIJINGXITUO。JIANGXITUOYEZAI40℃XIADANQICHUIGAN,YONG0.025 MLINSUANYANHUANCHONGYE(pH=7.0)DINGRONGCANZHAZHI1

mL,DAISHANGJICEDING。

SEPUTIAOJIAN:LabAlliance

PC-2000GAOXIAOYEXIANGSEPUPEI500XINGZIWAIJIANCEQI;

lanboSEPUZHU

:C18(4.6mm×150mm×5μm);

LIUDONGXIANG: JIACHUN/0.004M

LINSUANERQINGJIA(pH=4.5)=40/60;

JIANCEBOZHANG: 268 nm

2、ZHIJIEFA

FANGFAYUANLI:NIURUZHONGDEQINGMEISUJIAZAIβ-NEIXIANANMEIDEZUOYONGXIA,ZHUYAOSHENGCHENGQINGMEISAIZUOSUANJIA,BINGBANSUISHENGCHENGSHAOLIANGDEQINGMEIAN、QINGMEIQUANDENGWUZHI。JINGQUZHIFANG、DANBAIDENGQIANCHULIHOU,KEYIJIANGQINGMEISAIZUOSUANJIATIQUCHULAI,JINGGAOXIAOYEXIANGSEPUYIJIANCEQUERENQISHIFOUCUNZAI,CONGERPANDINGGAINIURUSHIFOUWEIRENZAO“WUKANGNAI”。

SHIYANBUZHOU:CHENGQU20 gSHIYANG,ZAI4 ℃、16000 rpmTIAOJIANXIALIXIN10 min。QUXIACENGQINGYE10 gYU50

mLSULIAOLIXINGUAN,JIARUWUSHUIYICHUN15 mL,ZHENDANGTIQU30 minHOULIXIN,QUQINGYEJING0.45

μmDELVMOGUOLV,YONGGAOXIAOYEXIANGSEPUYIJIANCE。

BIAOZHUNRONGYEPEIZHI:CHENGQU0.01 g(JINGQUEZHI0.001 g)QINGMEISUJIAYU100

mlRONGLIANGPINGZHONG,JIARU1mlSHISHOUKANGSHENGSUFENJIEJI,SHIWENFANGZHI2hSHIQINGMEISUJIAMEIJIEWANQUANHOUYONGQULIZISHUIDINGRONG,DEDAOQINGMEISAIZUOSUANJIABIAOZHUNRONGYE,NONGDU100

μg/mL。GENJUSHIJIQINGKUANGXISHIHOUSHIYONG。

SEPUTIAOJIAN:LabAlliance

PC-2000GAOXIAOYEXIANGSEPUPEI2000XINGZIWAIJIANCEQI;

lanboSEPUZHU :C18(4.6mm×150mm×5μm);

LIUDONGXIANG: JIACHUN/0.004

MLINSUANERQINGJIA(pH=4.5)=40/60;

检测波长: 230 nm

 
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